1.

For performing EMSA what type of gel should be used?(a) Native(b) Denaturing SDS-PAGE(c) Non-denaturing SDS PAGE(d) ZymographyThe question was asked in an interview for job.This key question is from Transcription in Eukaryotes : Transcription Factors in portion Gene Expression : Transcription Prokaryotes of Cytogenetics

Answer»

The correct choice is (C) Non-denaturing SDS PAGE

To ELABORATE: A denaturing SDS PAGE would BREAK the interactions between the FACTORS which is being studied. Thus, a non-denaturing SDS PAGE is used. A native page on the other hand would be less clear while zymography is UNRELATED.



Discussion

No Comment Found

Related InterviewSolutions