In an experiment you add growing concentration of the alpha amanitin. What will be the sequence in which RNA polymerases are affected if at all?(a) Pol I -> pol III , pol II unaffected(b) pol I->pol I ->pol III(c) pol III->pol II -> pol I(d) pol II -> pol III , pol I unaffectedI had been asked this question during an online exam.Question is from Transcription in Eukaryotes : RNA Polymerases topic in portion Gene Expression : Transcription Prokaryotes of Cytogenetics

In an experiment you add growing concentration of the alpha amanitin.

1.	In an experiment you add growing concentration of the alpha amanitin. What will be the sequence in which RNA polymerases are affected if at all?(a) Pol I -> pol III , pol II unaffected(b) pol I->pol I ->pol III(c) pol III->pol II -> pol I(d) pol II -> pol III , pol I unaffectedI had been asked this question during an online exam.Question is from Transcription in Eukaryotes : RNA Polymerases topic in portion Gene Expression : Transcription Prokaryotes of Cytogenetics
Answer» Right ANSWER is (d) pol II -> pol III , pol I unaffected Easiest EXPLANATION: RNA polymerase II is inactivated by very small level of alpha amanitin as low as 1 microgram PER ml while on the other hand pol III is affected at 10 MICROGRAMS per ml concentration. Polymerase I remain unaffected EVEN at very high alpha amanitin concentration.

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