In an experiment, you are trying to pull down the site where guanyl transferase binds by co-immunoprecipitation. You run a gel and autoradiograph to see a band denoting GSK-RNA pol II (phosphorylated). What will you see when you perform the same experiment with overexpression of GSK in one setup and overexpression of unlabelled GSK-RNA pol II in the other setup?(a) Band is observed in both cases(b) Band in 1^st case no band in 2^nd(c) No band in 1^st case and band in 2^nd(d) No band in both casesI got this question by my school principal while I was bunking the class.The origin of the question is Post-Transcriptional Modification in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics

In an experiment, you are trying to pull down the site where guanyl tr

1.	In an experiment, you are trying to pull down the site where guanyl transferase binds by co-immunoprecipitation. You run a gel and autoradiograph to see a band denoting GSK-RNA pol II (phosphorylated). What will you see when you perform the same experiment with overexpression of GSK in one setup and overexpression of unlabelled GSK-RNA pol II in the other setup?(a) Band is observed in both cases(b) Band in 1^st case no band in 2^nd(c) No band in 1^st case and band in 2^nd(d) No band in both casesI got this question by my school principal while I was bunking the class.The origin of the question is Post-Transcriptional Modification in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics
Answer» Correct option is (a) Band is OBSERVED in both cases The explanation is: There will not be any change in the intensity of the 1^st band as GT is not binding to GSK tag. The 2^nd case also doesn’t change the intensity of the band as GT BINDS to phosphorylated –GSK-RNA pol II, so no GT BOUND unlabelled RNA exists to compete with the labeled band and decrease its intensity.

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