In an experiment you mutate the consensus sequence at the -10 box. You observe the rate of transcription reduces. Now you make complementary mutation to the sigma factor. What will you observe?(a) Further decrease in transcription rate(b) Increase in transcription rate(c) Original transcription rate(d) Increase in rate of random transcriptionI have been asked this question by my school teacher while I was bunking the class.My question is based upon Transcription in Prokaryotes : Initiation in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics

In an experiment you mutate the consensus sequence at the -10 box. You

1.	In an experiment you mutate the consensus sequence at the -10 box. You observe the rate of transcription reduces. Now you make complementary mutation to the sigma factor. What will you observe?(a) Further decrease in transcription rate(b) Increase in transcription rate(c) Original transcription rate(d) Increase in rate of random transcriptionI have been asked this question by my school teacher while I was bunking the class.My question is based upon Transcription in Prokaryotes : Initiation in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics
Answer» RIGHT answer is (c) Original transcription rate To explain: Complementary mutation in sigma factor helps to restore its -10 box recognition CAPACITY which RESTORES NORMAL transcription rate. However, to IMPROVE the rate further both the -10 and -35 box sequences should be made more like the consensus.

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