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In what sequence are the serine residues in RNA pol II phosphorylated?(a) 5->2->7(b) 2->5->7(c) 2->7->5(d) 7->2->5I have been asked this question in semester exam.My query is from Transcription in Eukaryotes : RNA Polymerases topic in section Gene Expression : Transcription Prokaryotes of Cytogenetics

Answer»

Correct option is (c) 2->7->5

The explanation is: The phosphorylation is at the 2^nd serine residue with the HELP of the TFIIH, and then during elongation the phosphorylation is at the 5^th serine residue. Between these two there is a short PHASE of phosphorylation at the 7^th serine.



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