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Melting of DNA would lead to _________________(a) Increase in UV absorption(b) Increase in Fluorescence(c) Decrease in UV absorption(d) Decrease in fluorescenceThe question was asked in my homework.This question is from Transcription in Prokaryotes : Elongation in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics

Answer»

The correct choice is (a) INCREASE in UV absorption

Easy EXPLANATION: MELTING is the separation of the two strands of DNA which CAUSES an increase in the amount of UV RAYS absorbed by the DNA. This is known as Hyperchromic shift. It has no effect on florescence of DNA.



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