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The concept of transmembrane proteins was obtained from the results of which technique?(a) Freeze-fraction replication(b) Freeze-fracture replication(c) Fraction replication(d) None of the mentionedThis question was posed to me in a national level competition.My question is from Cellular Membranes topic in portion Cellular Membranes of Cell Biology

Answer»

The correct option is (b) Freeze-fracture REPLICATION

The explanation: The concept that transmembrane proteins PENETRATE through the membrane was obtained from the results of freeze-fracture replication. In this procedure, a FROZEN solid tissue is fractured with a knife blade and metals are deposited on their exposed surfaces to form shadowed REPLICA. This is viewed under ELECTRON microscope and it looks like a path strewn with pebbles, called as membrane-associated particles.



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