Theoretical plates are used to ___________(a) Determine the thickness of the mobile phase(b) Determine the thickness of the stationary phase(c) Estimate the efficiency of the column(d) Measure the distribution of the analyte between mobile and stationary phasesThis question was addressed to me in a job interview.Question is from Chromatography in division Unit Operations of Bioprocess Engineering

Theoretical plates are used to ___________(a) Determine the thickness

1.	Theoretical plates are used to ___________(a) Determine the thickness of the mobile phase(b) Determine the thickness of the stationary phase(c) Estimate the efficiency of the column(d) Measure the distribution of the analyte between mobile and stationary phasesThis question was addressed to me in a job interview.Question is from Chromatography in division Unit Operations of Bioprocess Engineering
Answer» The correct option is (C) Estimate the efficiency of the column Best explanation: The PLATE model SUPPOSES that the chromatographic column contains a large number of separate LAYERS, called THEORETICAL plates. Separate equilibrations of the sample between the stationary and mobile phase occur in these “plates”.

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