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Which of the following centrifugation is used to separate certain organelles from whole cell?(a) Rate-zonal centrifugation(b) Normal centrifugation(c) Differential centrifugation(d) Isopycnic centrifugationThe question was asked in exam.This is a very interesting question from Centrifugation in section Unit Operations of Bioprocess Engineering

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Right OPTION is (c) Differential centrifugation

Easy explanation: The simplest form of separation by centrifugation is differential centrifugation, sometimes called differential pelleting. Particles of different densities or sizes in a suspension will sediment at different rates, with the larger and denser particles sedimenting faster. These sedimentation rates can be increased by using centrifugal force. A suspension of CELLS subjected to a series of increasing centrifugal force cycles will yield a series of PELLETS containing cells of decreasing sedimentation rate.

Differential centrifugation is a common PROCEDURE in microbiology and cytology USED to separate certain organelles from whole cells for further analysis of specific parts of cells.



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