What is the direction in which the transcript produced by RNA polymerase grows?(a) 3’->5’ direction on 3’->5’ strand(b) 5’->3’ direction on 5’->3’ strand(c) 3’->5’ direction on 5’->3’ strand(d) 5’->3’ direction on 3’->5’ strandI got this question in an interview for job.My question comes from Transcription in Prokaryotes : Initiation in division Gene Expression : Transcription Prokaryotes of Cytogenetics

What is the direction in which the transcript produced by RNA polymera

1.	What is the direction in which the transcript produced by RNA polymerase grows?(a) 3’->5’ direction on 3’->5’ strand(b) 5’->3’ direction on 5’->3’ strand(c) 3’->5’ direction on 5’->3’ strand(d) 5’->3’ direction on 3’->5’ strandI got this question in an interview for job.My question comes from Transcription in Prokaryotes : Initiation in division Gene Expression : Transcription Prokaryotes of Cytogenetics
Answer» Correct option is (d) 5’->3’ DIRECTION on 3’->5’ strand Easy explanation: In the process of RNA synthesis there is a short phase of hybrid DNA-RNA FORMATION. This is possible only when two strands are oppositely ORIENTED. As 3’-OH is necessary for adding new nucleotides, RNA is synthesized from 5’->3’ direction, so the template MUST be 3’->5’ oriented.

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