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What useful information can be found from a Van Deemter plot?(a) Optimum column length(b) Optimum column temperature(c) Optimum mobile phase flow rate(d) The selectivity factorI got this question in examination.The origin of the question is Chromatography in chapter Unit Operations of Bioprocess Engineering

Answer»

The correct answer is (a) Optimum column LENGTH

Explanation: The Van Deemter equation is an empirical formula describing the relationship between plate HEIGHT (H, the length needed for one theoretical plate) which is a measure of column efficiency, and linear velocity (µ) . Smaller plate height values correspond to greater peak efficiencies, as more plates, or analyte partitioning, can occur over a fixed length of a column. The Van Deemter equation is governed by three cumulative terms: (A) eddy diffusion, (B) LONGITUDINAL diffusion, and (C) mass transfer. A loss in peak efficiency can be observed as a wider analyte band, and therefore, these three terms can also be VIEWED as factors that CONTRIBUTE to band broadening.


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