Which cation is placed in the catalytic subunit of RNA polymerase?(a) Zn^2+(b) Mn^2+(c) Mg^2+(d) Fe^3+The question was asked in an online interview.My question is based upon Transcription in Prokaryotes : Initiation topic in portion Gene Expression : Transcription Prokaryotes of Cytogenetics

Which cation is placed in the catalytic subunit of RNA polymerase?(a)

1.	Which cation is placed in the catalytic subunit of RNA polymerase?(a) Zn^2+(b) Mn^2+(c) Mg^2+(d) Fe^3+The question was asked in an online interview.My question is based upon Transcription in Prokaryotes : Initiation topic in portion Gene Expression : Transcription Prokaryotes of Cytogenetics
Answer» Right OPTION is (c) Mg^2+ The explanation is: Magnesium ion is MANDATORY for the catalytic ACTION as it shields the NEGATIVE charge of the phosphate groups of rNTPs and also promotes the nucleophilic attack by the 3’-OH group. Manganese, zinc and iron are part of other biologically important enzymes.

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