Which of the following condition is of reverse phase chromatography?(a) The mobile phase is non-polar and stationary phase is polar(b) The mobile phase is polar and stationary phase is non-polar(c) Both the mobile phase and stationary phase are organic(d) Both the mobile phase and stationary phase are inorganicI got this question in an international level competition.My question is based upon Chromatography in chapter Unit Operations of Bioprocess Engineering

Which of the following condition is of reverse phase chromatography?(a

1.	Which of the following condition is of reverse phase chromatography?(a) The mobile phase is non-polar and stationary phase is polar(b) The mobile phase is polar and stationary phase is non-polar(c) Both the mobile phase and stationary phase are organic(d) Both the mobile phase and stationary phase are inorganicI got this question in an international level competition.My question is based upon Chromatography in chapter Unit Operations of Bioprocess Engineering
Answer» Correct answer is (b) The mobile PHASE is polar and stationary phase is non-polar The BEST I can EXPLAIN: In reversed phase chromatography, the mobile phase is polar (like WATER), and the stationary phase is non-polar while in NORMAL phase chromatography, the mobile phase is non-polar (100% organic), and the stationary phase is polar.

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