Which of the following will increase transcription?(a) Shielding positive charge of DNA(b) Shielding positive charge of histones(c) Shielding promoter for polymerase binding(d) Shielding termination regionThis question was addressed to me in my homework.My enquiry is from Transcription in Eukaryotes : Chromatin Remodelling in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics

Which of the following will increase transcription?(a) Shielding posit

1.	Which of the following will increase transcription?(a) Shielding positive charge of DNA(b) Shielding positive charge of histones(c) Shielding promoter for polymerase binding(d) Shielding termination regionThis question was addressed to me in my homework.My enquiry is from Transcription in Eukaryotes : Chromatin Remodelling in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics
Answer» The CORRECT option is (C) Shielding promoter for polymerase binding To elaborate: Histone binds to the negatively charged DNA due to its POSITIVE CHARGES. On DNA binding those regions of the DNA are not available for transcription. Thus, shielding the positive charge would loosen this binding and ENABLE more transcription.

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