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Which of these sequences will mark a site of polyadenylation?(a) AAUAAA—-GGUUUGU——-CA——-(b) UUUGUGUG—–CA—-AUUAAA(c) AAUAAA—–CA—-UUUUGUGU(d) CA—–AAUAAA——UUUUGUGUGUThe question was asked in a national level competition.My doubt stems from Post-Transcriptional Modification in division Gene Expression : Transcription Prokaryotes of Cytogenetics

Answer»

Correct answer is (c) AAUAAA—–CA—-UUUUGUGU

Explanation: The cleavage sequence has a CONSENSUS of AAUAAA sequence towards the 5’ and a G/U box towards the 3’ end and a CA DINUCLEOTIDE in the middle. The cleavage is just after 3’ to the CA.



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