Which would be an appropriate method to detect the core promoter regions in a eukaryotic gene?(a) Footprint(b) Sequencing(c) Linker scanning(d) S1 endonucleaseI got this question in semester exam.My question is from Transcription in Eukaryotes : Activators and Repressors in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics

Which would be an appropriate method to detect the core promoter regio

1.	Which would be an appropriate method to detect the core promoter regions in a eukaryotic gene?(a) Footprint(b) Sequencing(c) Linker scanning(d) S1 endonucleaseI got this question in semester exam.My question is from Transcription in Eukaryotes : Activators and Repressors in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics
Answer» Correct ANSWER is (c) Linker scanning To explain: In linker scanning method a SMALL segment of the DNA is removed from PROMOTER region and replaced with a RANDOM segment to see its effect on the transcription rate. Thus, as absence of promoter would lead to less transcription this method can be used to detect promoter ELEMENTS.

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