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You artificially increase the level of σ^32 in an E. coli culture. What will you expect to see?(a) rpo H transcription permanently inhibited(b) cdc transcription constitutively turned on(c) rpo H temporarily activated(d) cdc 6 permanently turned offThe question was posed to me in unit test.I'm obligated to ask this question of Transcription in Prokaryotes : Sigma Factor topic in portion Gene Expression : Transcription Prokaryotes of Cytogenetics

Answer»

Correct option is (c) rpo H TEMPORARILY activated

Easy explanation: σ^32 is necessary for transcribing genes like rpo H that helps in surviving heat shock while it doesn’t ACT on cdc6 that has a role in REPLICATION. As σ^32 is unstable it temporarily turns on rho H TILL the concentration RATIO of σ^32 and σ^70 returns to normal.



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