You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?(a) Moderate concentration of RNA pol II will precipitate(b) RNA pol II that precipitated will be contaminated with pol III(c) High amount of RNA pol II will precipitate(d) No RNA pol II will precipitateThis question was addressed to me in semester exam.Enquiry is from Transcription in Eukaryotes : RNA Polymerases in section Gene Expression : Transcription Prokaryotes of Cytogenetics

You designed an antibody against phosphorylated CTD of RNA polymerase

1.	You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?(a) Moderate concentration of RNA pol II will precipitate(b) RNA pol II that precipitated will be contaminated with pol III(c) High amount of RNA pol II will precipitate(d) No RNA pol II will precipitateThis question was addressed to me in semester exam.Enquiry is from Transcription in Eukaryotes : RNA Polymerases in section Gene Expression : Transcription Prokaryotes of Cytogenetics
Answer» The CORRECT answer is (d) No RNA pol II will precipitate The best explanation: Lac operon is a prokaryotic gene not recognized by eukaryotic RNA polymerase. Thus it will not be transcribing. Non-Transcribing RNA pol II doesn’t have phosphorylated residue in CTD, so it can’t be immunoprecipitated with the given ANTIBODY.

Discussion

No Comment Found

Related InterviewSolutions

PAP has a processivity of ___________(a) 5-10 bases(b) 10-20 bases(c) 50-100 bases(d) 100-250 basesI got this question by my college director while I was bunking the class.Query is from Post-Transcriptional Modification topic in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics
Which of the following is not a common mode of histone modification in eukaryotes?(a) Methylation(b) Phosphortlation(c) Sulphonation(d) UbiquitinylationThis question was posed to me in final exam.Question is taken from Transcription in Eukaryotes : Chromatin Remodelling in portion Gene Expression : Transcription Prokaryotes of Cytogenetics
Which of these class III promoter type resemble class II?(a) Type I(b) Type II(c) Type III(d) Type IVThe question was posed to me in class test.My query is from Transcription in Eukaryotes : Activators and Repressors topic in section Gene Expression : Transcription Prokaryotes of Cytogenetics
How many of eukaryotic RNA polymerase subunits are common for all three polymerases?(a) 3(b) 5(c) 10(d) 7This question was addressed to me during an online interview.Question is taken from Transcription in Eukaryotes : RNA Polymerases topic in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics
Which of the following is retained in the C complex of splicosome?(a) U5(b) U4(c) U2AF(d) U1The question was posed to me in an interview for internship.My doubt stems from Post-Transcriptional Modification topic in portion Gene Expression : Transcription Prokaryotes of Cytogenetics
5’______ and 3’ ____ is the sequence for two ends of an intron.(a) GA TC(b) GU AG(c) AG TA(d) GA ATI have been asked this question in unit test.This interesting question is from Post-Transcriptional Modification in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics
The post transcriptional modifications are done after the transcript is completely transcribed. State whether the statement is true or false.(a) True(b) FalseI have been asked this question during an online interview.This interesting question is from Post-Transcriptional Modification topic in portion Gene Expression : Transcription Prokaryotes of Cytogenetics
Which of these SIR proteins are having HDAC activity?(a) SIR1(b) SIR2(c) SIR3(d) SIR4I got this question during a job interview.This intriguing question originated from Transcription in Eukaryotes : Gene Silencing at Telomere topic in portion Gene Expression : Transcription Prokaryotes of Cytogenetics
If a parent yeast is of mating type a, what will be the mating type of its bud?(a) a(b) alpha(c) rho(d) a-alphaThe question was posed to me in examination.Origin of the question is Transcription in Eukaryotes : Gene Silencing at Telomere topic in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics
In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1^st set you deleted 94 amino acids from the C-terminus and in the 2^nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?(a) 94 amino acid mutant(b) 265^th amino acid mutant(c) Both will have similar negative effect(d) No visible effect on rate of transcriptionI had been asked this question during an interview.I'm obligated to ask this question of Transcription in Prokaryotes : Initiation topic in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics

Discussion

No Comment Found

Related InterviewSolutions

Reply to Comment