You transfer the DAM gene to the telomere of yeast. What will be your expected result?(a) Methylation at the A sites in specific sequence(b) Methylation at the C sites in specific sequence(c) No methylation at the A sites in specific sequence(d) No methylation at the C sites in specific sequenceThe question was asked at a job interview.I want to ask this question from Transcription in Eukaryotes : Gene Silencing at Telomere topic in section Gene Expression : Transcription Prokaryotes of Cytogenetics

You transfer the DAM gene to the telomere of yeast. What will be your

1.	You transfer the DAM gene to the telomere of yeast. What will be your expected result?(a) Methylation at the A sites in specific sequence(b) Methylation at the C sites in specific sequence(c) No methylation at the A sites in specific sequence(d) No methylation at the C sites in specific sequenceThe question was asked at a job interview.I want to ask this question from Transcription in Eukaryotes : Gene Silencing at Telomere topic in section Gene Expression : Transcription Prokaryotes of Cytogenetics
Answer» The correct answer is (c) No methylation at the A sites in specific sequence The BEST EXPLANATION: DAM is a METHYLASE that methylates the A of GATC sequence. When expressed in Yeast it methylated the yeast DNA, however, in telomere the EXPRESSION is blocked by the telomere silencing effect. Thus, we don’t get methylated DNA.

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