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Gel-filtration chromatography separates proteins based on their ___________________(a) pH(b) temperature(c) morphology(d) effective sizeThe question was posed to me in an internship interview.I want to ask this question from Protein Techniques in division Techniques in Cell Biology of Cell Biology

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Right answer is (d) effective SIZE

To explain: Gel-filtration chromatography separates the proteins BASED on their effective size (hydrodynamic radius). The COLUMN material consists of cross-linked polysaccharides like AGAROSE and dextrans.



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