If give a segment – 5’CTGACCAUGGCCTAGAUGCCTCT, which AUG will the eukaryotic ribosome choose as initiation site?(a) Any at random(b) First(c) Second(d) NoneI had been asked this question in an international level competition.This key question is from Initiation in portion Gene Expression : Translation of Cytogenetics

If give a segment – 5’CTGACCAUGGCCTAGAUGCCTCT, which AUG will the

1.	If give a segment – 5’CTGACCAUGGCCTAGAUGCCTCT, which AUG will the eukaryotic ribosome choose as initiation site?(a) Any at random(b) First(c) Second(d) NoneI had been asked this question in an international level competition.This key question is from Initiation in portion Gene Expression : Translation of Cytogenetics
Answer» Right ANSWER is (b) First Explanation: The ribosome scans the mRNA from 5’ end to the 3’ end searching for a Kozak consensus sequence to start at. Here, the 1^st AUG indeed has a purin at -3 and +4 position so it is a good INITIATION site, the ribosome will not LOOK beyond to the 2^nd AUG(WEAKER) and start initiation.

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