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In an experiment if you add poly A oigon-dNTP in a nucleotide free cell lysate. Which of the labeled amino acid do you expect to be incorporated in the peptide so formed?(a) Lys(b) Phe(c) Pro(d) LeuThis question was posed to me in unit test.My enquiry is from Genetic Code topic in portion Gene Expression : Translation of Cytogenetics

Answer»

Correct CHOICE is (b) Phe

For explanation I WOULD say: While poly A oligo-rNTP would incorporate lysine, due to repeated AAA CODON, oligo-dNTP actually makes poly U oligo-rNTP, having codon UUU that codes for PHENYL alanine.



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