In an experiment you grow some cells in a media containing heavy isotopes of C and N. Then you transfer then to a lighter media. If you centrifuge the ribosomes so obtained after an hour how many bands will you expect?(a) 1(b) 2(c) 3(d) 4The question was asked in an internship interview.Enquiry is from Ribosome as Ribozyme in portion Gene Expression : Translation of Cytogenetics

In an experiment you grow some cells in a media containing heavy isoto

1.	In an experiment you grow some cells in a media containing heavy isotopes of C and N. Then you transfer then to a lighter media. If you centrifuge the ribosomes so obtained after an hour how many bands will you expect?(a) 1(b) 2(c) 3(d) 4The question was asked in an internship interview.Enquiry is from Ribosome as Ribozyme in portion Gene Expression : Translation of Cytogenetics
Answer» The CORRECT choice is (d) 4 To explain I WOULD say: You will OBTAIN 4 BANDS corresponding to heavy 50 and 30S SUBUNIT, heavy 50S and light 30S subunit, heavy 30S and light 50S subunit and light 50S and 30S subunit respectively.

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