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In an experiment you take a DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?(a) Dna a, Dna b, HU, SSB, topoisomerase I, polymerase I(b) Dna a, Dna b, HU, SSB, polymerase III(c) Dna a, Dna b,Dna c, HU ,SSBpolymerase I(d) Dna a, Dna b, Dna c, HU, SSB, polymerase IIII have been asked this question by my school principal while I was bunking the class.I'd like to ask this question from DNA Replication Prokaryotes in portion DNA Replication of Cytogenetics

Answer»

Right option is (C) Dna a, Dna b,Dna c, HU ,SSBpolymerase I

To explain I would say: DNA pol I has primase activity which is mandatory for starting DNA replication. As pol III lacks primase activity it will not be able to initiate replication. Also in vitro(specially in open DNA strand) topoisomerase doesn’t have MUCH role to play but Dna c is a must for replication as it LOADS the helicase.



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