In ion-exchange chromatography, proteins bound to the resin can be displaced by increasing the _____________________(a) strength of ionic buffer(b) size of sample(c) column volume(d) column widthThis question was addressed to me in class test.I would like to ask this question from Protein Techniques in chapter Techniques in Cell Biology of Cell Biology

In ion-exchange chromatography, proteins bound to the resin can be dis

1.	In ion-exchange chromatography, proteins bound to the resin can be displaced by increasing the _____________________(a) strength of ionic buffer(b) size of sample(c) column volume(d) column widthThis question was addressed to me in class test.I would like to ask this question from Protein Techniques in chapter Techniques in Cell Biology of Cell Biology
Answer» The correct OPTION is (a) strength of IONIC BUFFER For explanation: In ion-exchange chromatography, the proteins bind reversibly to the INERT resin through ionic BONDS. To displace and eventually elute the bound proteins the ionic strength of buffer can be increased.

Discussion

No Comment Found

Related InterviewSolutions

At the critical drying point, there is no surface tension between ________________(a) gas and oil(b) gas and liquid(c) liquid and liquid(d) oil and liquidThis question was posed to me in an interview for job.This intriguing question originated from Techniques in portion Techniques in Cell Biology of Cell Biology
In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?(a) Western blotting(b) Southern blotting(c) Isopycnic centrifugation(d) SpectrophotometryI got this question in exam.My query is from Nucleic-acid Techniques topic in section Techniques in Cell Biology of Cell Biology
The process of introducing DNA into cells is called ____________________(a) blotting(b) conjugation(c) transfection(d) conductionThis question was posed to me in an internship interview.This question is from Techniques in section Techniques in Cell Biology of Cell Biology
Synchrotrons generate _____________________(a) Peptides(b) X rays(c) Infrared rays(d) CarcinogensThis question was addressed to me during an internship interview.Enquiry is from Techniques topic in division Techniques in Cell Biology of Cell Biology
Which is the main ingredient in the sample preparation of mass spectrometry?(a) papain(b) pepsin(c) vinculin(d) trypsinI had been asked this question in semester exam.The doubt is from Techniques topic in section Techniques in Cell Biology of Cell Biology
In gel-filtration chromatography, the proteins ______________________(a) bind to the column(b) diffuse through the column(c) denature(d) vaporizeThis question was posed to me in an online quiz.My question is based upon Protein Techniques in portion Techniques in Cell Biology of Cell Biology
Image formation in electron microscope is based on ___________________________(a) column length(b) electron number(c) differential scattering(d) specimen sizeThis question was posed to me in an online interview.The origin of the question is Techniques topic in section Techniques in Cell Biology of Cell Biology
The resolution attainable with standard TEM is less than the theoretical value.(a) True(b) FalseI had been asked this question in my homework.I'm obligated to ask this question of Techniques topic in portion Techniques in Cell Biology of Cell Biology
Which of the following molecules can be analyzed using a northern blot?(a) RNA(b) Carbohydrates(c) Proteins(d) DNAThe question was asked in exam.This question is from Nucleic-acid Techniques topic in portion Techniques in Cell Biology of Cell Biology
Which of the following can not be used as a fixative?(a) formaldehyde(b) acetic acid(c) alcohol(d) vitamin B12The question was posed to me by my school principal while I was bunking the class.My doubt is from Techniques in division Techniques in Cell Biology of Cell Biology

Discussion

No Comment Found

Related InterviewSolutions

Reply to Comment