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In purification steps of nucleic-acids, phenol is used for _______________________(a) deproteinization(b) denaturation(c) lowering pH(d) lowering viscosityI got this question by my school principal while I was bunking the class.The origin of the question is Nucleic-acid Techniques topic in section Techniques in Cell Biology of Cell Biology

Answer»

The correct OPTION is (a) deproteinization

Easiest explanation: After the release of nuclear CONTENT of a cell, the next STEP is to free the nucleic acids from proteins and RNA. Phenol is used to deproteinize the SAMPLE; a mixture of phenol and chloroform may also be used.



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