Proteins interacting with specific substances can be separated using ___________________(a) ion-exchange chromatography(b) paper chromatography(c) affinity chromatography(d) gel-filtration chromatographyThe question was asked in a national level competition.This intriguing question originated from Protein Techniques in section Techniques in Cell Biology of Cell Biology

Proteins interacting with specific substances can be separated using _

1.	Proteins interacting with specific substances can be separated using ___________________(a) ion-exchange chromatography(b) paper chromatography(c) affinity chromatography(d) gel-filtration chromatographyThe question was asked in a national level competition.This intriguing question originated from Protein Techniques in section Techniques in Cell Biology of Cell Biology
Answer» Correct option is (C) affinity chromatography Best explanation: The proteins participating in interactions such as ligand-ligand, enzyme-substrate etc. can be PURIFIED using the affinity chromatography. In this technique, an interacting molecule is covalently attached to the INERT MATRIX.

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