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The resolution attained by a microscope is limited by _____________________(a) diffraction(b) refraction(c) reflection(d) retractionThis question was posed to me by my school principal while I was bunking the class.Asked question is from Techniques topic in chapter Techniques in Cell Biology of Cell Biology

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Correct CHOICE is (a) diffraction

To elaborate: The resolution attained by a MICROSCOPE is LIMITED by diffraction. DUE to diffraction, the light EMANATING from a sample can only be seen as a disk rather than a point of image.



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