The temperature cycles in a polymerase chain reaction are in the order __________________(a) 95°, 60°, 72°(b) 60°, 72°, 95°(c) 72°, 60°, 95°(d) 95°, 72°, 60°This question was posed to me by my college director while I was bunking the class.My question is based upon Techniques topic in division Techniques in Cell Biology of Cell Biology

The temperature cycles in a polymerase chain reaction are in the order

1.	The temperature cycles in a polymerase chain reaction are in the order __________________(a) 95°, 60°, 72°(b) 60°, 72°, 95°(c) 72°, 60°, 95°(d) 95°, 72°, 60°This question was posed to me by my college director while I was bunking the class.My question is based upon Techniques topic in division Techniques in Cell Biology of Cell Biology
Answer» The CORRECT answer is (a) 95°, 60°, 72° Easiest explanation: Three different TEMPERATURES are reached during the polymerase CHAIN REACTION to facilitate the denaturation and hybridization of the DNA strands. First the temperature is elevated to 95°C, then lowered to 60°C and then again increased to 72°C.

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