What was the unnatural experimental condition which proved to be a criticism in Robert Traut and Munro experiment?(a) Use of low Mg^2+ concentration(b) Use of 33% methanol(c) Use of purified ribosome(d) Use of puromycinThis question was posed to me in unit test.My question is from Ribosome as Ribozyme topic in division Gene Expression : Translation of Cytogenetics

What was the unnatural experimental condition which proved to be a cri

1.	What was the unnatural experimental condition which proved to be a criticism in Robert Traut and Munro experiment?(a) Use of low Mg^2+ concentration(b) Use of 33% methanol(c) Use of purified ribosome(d) Use of puromycinThis question was posed to me in unit test.My question is from Ribosome as Ribozyme topic in division Gene Expression : Translation of Cytogenetics
Answer» Right option is (a) Use of low Mg^2+ concentration The BEST I can explain: The condition of 33% methanol is not usual for a celluar interior. Thus Robert Traut and MUNRO’s using 33% methanol (or ethanol) to carry out peptidyl transfer ACTIVITY was criticized.

Discussion

No Comment Found

Related InterviewSolutions

In an experiment you delete a part of the 5’ UTR of transferring mRNA, what will be its effect on iron sensitivity?(a) It will be able to sense iron changes better(b) It will not be able to sense the iron changes(c) No change(d) It will have leaky sensitivityThis question was posed to me during an internship interview.The above asked question is from Control of Translation topic in division Gene Expression : Translation of Cytogenetics
When the intracellular iron level is low, the turnover of transferrin mRNA is________(a) Increased(b) Decreased(c) Kept normal(d) Doesn’t effectThe question was asked in homework.This interesting question is from Control of Translation in portion Gene Expression : Translation of Cytogenetics
Which of these factors can recognize the UAA stop codon?(a) RF1(b) RF3(c) RRF(d) Ef-GThe question was posed to me in an internship interview.My question is taken from Elongation and Termination topic in division Gene Expression : Translation of Cytogenetics
Translation of which sigma factor is dependent on a genetic thermostat?(a) Sigma 70(b) Sigma 32(c) Sigma E(d) Sigma SI got this question by my school principal while I was bunking the class.Question is from Control of Translation topic in division Gene Expression : Translation of Cytogenetics
Phosphorylation of eIF4E-BP will affect translation in what way?(a) Increase(b) Decrease(c) No effect(d) There is no such factorI have been asked this question in an internship interview.Question is from Initiation topic in portion Gene Expression : Translation of Cytogenetics
Binding of RF3.GTP to the ribosome helps in release of the ____________(a) Peptide(b) Release factor 1(c) tRNA(d) RibosomeThe question was posed to me in quiz.The doubt is from Elongation and Termination topic in division Gene Expression : Translation of Cytogenetics
According to the hybrid state model, if we consider the hybrid state of a tRNA such that its base is bound to the P site of 30S subunit, where will its head be bound?(a) A site of 50S(b) P site of 50S(c) E site of 50S(d) UnboundI got this question during an interview.Enquiry is from Elongation and Termination in chapter Gene Expression : Translation of Cytogenetics
Which initiation factor in eukaryotes recognizes the 7-M-G cap?(a) eIF4G(b) eIF4B(c) eIF4E(d) eIF4A1This question was addressed to me by my school teacher while I was bunking the class.Asked question is from Initiation in division Gene Expression : Translation of Cytogenetics
IRE-BP does not bind to _______________(a) IRES(b) Stem loop in 5’ end of Ferritin(c) Stem loop in 3’ end of Transferrin(d) AU rich regions in IREThe question was posed to me in unit test.This is a very interesting question from Translation in Eukaryotes topic in section Gene Expression : Translation of Cytogenetics
Which of the following will effectively cause normal translation from an initiation AUG, blocked by a strong AUG upstream?(a) Hairpin loop near the 1^st AUG(b) No measure required(c) In frame stop codon between two AUGs(d) Deletion of the GAP between the two AUGsI had been asked this question in an online quiz.Origin of the question is Translation in Eukaryotes in portion Gene Expression : Translation of Cytogenetics

Discussion

No Comment Found

Related InterviewSolutions

Reply to Comment