Which enzyme is active at 72° in the polymerase chain reaction?(a) isomerase(b) exonuclease(c) polymerase(d) endonucleaseI have been asked this question during an internship interview.My query is from Techniques topic in division Techniques in Cell Biology of Cell Biology

Which enzyme is active at 72° in the polymerase chain reaction?(a) is

1.	Which enzyme is active at 72° in the polymerase chain reaction?(a) isomerase(b) exonuclease(c) polymerase(d) endonucleaseI have been asked this question during an internship interview.My query is from Techniques topic in division Techniques in Cell Biology of Cell Biology
Answer» Right CHOICE is (c) polymerase Easy explanation: Taq polymerase, derived from the thermophilic BACTERIA Thermus aquaticus is active at 72°. The enzyme is responsible for synthesizing the complementary DNA strand by ADDING NUCLEOTIDES to the 3’ end of the primers.

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