Which of the following can be used as a temperature-sensitive mutant to study the endomembrane system?(a) Vesicular Stomatitis Virus(b) Human Immunodeficiency Virus(c) Tobacco Mosaic Virus(d) RetrovirusThe question was asked in a job interview.This intriguing question originated from Cytoplasmic Membrane Systems topic in division Cytoplasmic Membrane Systems of Cell Biology

Which of the following can be used as a temperature-sensitive mutant t

1.	Which of the following can be used as a temperature-sensitive mutant to study the endomembrane system?(a) Vesicular Stomatitis Virus(b) Human Immunodeficiency Virus(c) Tobacco Mosaic Virus(d) RetrovirusThe question was asked in a job interview.This intriguing question originated from Cytoplasmic Membrane Systems topic in division Cytoplasmic Membrane Systems of Cell Biology
Answer» CORRECT ANSWER is (a) Vesicular Stomatitis Virus To explain I would say: VSVG is a viral gene of the Vesicular Stomatitis Virus, which is fused with the green fluorescent protein (GFP) to study the pathway of VSVG protein. In this case, a VSV with MUTANT VSVG protein can be used that can only grow at lower temperatures than NORMAL; CALLED the temperature-sensitive mutant.

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