1.

Which of the following is a Sanger’s reagent?(a) 1-fluoro-2, 4-dinitrobenzene(b) 1-fluoro-2, 3-dinitrobenzene(c) 1-fluoro-2, 4-trinitrobenzene(d) 1-fluoro-2, 3-trinitrobenzeneThe question was asked in semester exam.This intriguing question originated from Protein Sequences and Evolution topic in division Amino Acids, Peptides and Proteins of Biochemistry

Answer»

The CORRECT choice is (a) 1-fluoro-2, 4-dinitrobenzene

For EXPLANATION I WOULD SAY: To identify the amino-terminal amino acid residue, SANGER developed the reagent 1-fluoro-2, 4-dinitrobenzene (FDNB).



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