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Which of the following is used in PAGE to prevent the mixing of the sample with running buffer?(a) ethanol(b) methanol(c) chloroform(d) sucroseThe question was posed to me by my college director while I was bunking the class.The origin of the question is Techniques in chapter Techniques in Cell Biology of Cell Biology

Answer»

Right CHOICE is (d) sucrose

The best I can explain: For polyacrylamide gel electrophoresis (PAGE), the PROTEIN samples are PREPARED in a solution of sucrose or glycerol to PROVIDE density. This prevents the mixing of protein samples with the running buffer.



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