Which of the following statements is true about affinity chromatography?(a) During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first(b) During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last(c) During the separation of a mixture of proteins, the protein which binds to ligand is eluted first(d) Unwanted proteins are eluted by ligand solutionI got this question during an interview.Enquiry is from Working with Proteins in chapter Amino Acids, Peptides and Proteins of Biochemistry

Which of the following statements is true about affinity chromatograph

1.	Which of the following statements is true about affinity chromatography?(a) During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first(b) During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last(c) During the separation of a mixture of proteins, the protein which binds to ligand is eluted first(d) Unwanted proteins are eluted by ligand solutionI got this question during an interview.Enquiry is from Working with Proteins in chapter Amino Acids, Peptides and Proteins of Biochemistry
Answer» The CORRECT answer is (a) During the separation of a mixture of proteins, the protein which does not bind to LIGAND is eluted first To explain I WOULD SAY: After proteins that do not bind to the ligand are washed through the column, the bound protein of particular INTEREST is eluted.

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