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InterviewSolution
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Which of the following statements is true about affinity chromatography?(a) During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first(b) During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last(c) During the separation of a mixture of proteins, the protein which binds to ligand is eluted first(d) Unwanted proteins are eluted by ligand solution |
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Answer» The correct answer is (a) During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first To explain I would say: After proteins that do not bind to the ligand are washed through the column, the bound protein of particular interest is eluted. |
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