Which of the following statements is true about size-exclusion chromatography?(a) During the separation of a mixture of proteins, protein with smallest molecular weight is eluted first(b) During the separation of a mixture of proteins, protein with largest molecular weight is eluted first(c) During the separation of a mixture of proteins, protein with largest molecular weight is eluted last(d) During the separation of a mixture of proteins, protein with largest molecular weight flow around the beadsI got this question during a job interview.My doubt is from Working with Proteins in chapter Amino Acids, Peptides and Proteins of Biochemistry

Which of the following statements is true about size-exclusion chromat

1.	Which of the following statements is true about size-exclusion chromatography?(a) During the separation of a mixture of proteins, protein with smallest molecular weight is eluted first(b) During the separation of a mixture of proteins, protein with largest molecular weight is eluted first(c) During the separation of a mixture of proteins, protein with largest molecular weight is eluted last(d) During the separation of a mixture of proteins, protein with largest molecular weight flow around the beadsI got this question during a job interview.My doubt is from Working with Proteins in chapter Amino Acids, Peptides and Proteins of Biochemistry
Answer» Correct choice is (b) During the separation of a MIXTURE of proteins, protein with largest molecular weight is eluted first For explanation I would say: Larger proteins migrate faster than the SMALLER ones because they are too large to enter the pores in the BEADS.

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