Which technique can be used to separate ^15N and ^14N?(a) Rate-zonal centrifugation(b) Isopycnic centrifugation(c) HPLC(d) Mass spectrometryThis question was addressed to me in an interview for job.This question is from Nucleic-acid Techniques in section Techniques in Cell Biology of Cell Biology

Which technique can be used to separate ^15N and ^14N?(a) Rate-zonal c

1.	Which technique can be used to separate ^15N and ^14N?(a) Rate-zonal centrifugation(b) Isopycnic centrifugation(c) HPLC(d) Mass spectrometryThis question was addressed to me in an interview for job.This question is from Nucleic-acid Techniques in section Techniques in Cell Biology of Cell Biology
Answer» The correct option is (b) Isopycnic centrifugation Easy explanation: Isopycnic or equilibrium centrifugation can be used to separate nucleic ACIDS having different base COMPOSITION and EVEN different isotopes of NITROGEN such as ^15N and ^14N.

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Which technique can be used to separate ^15N and ^14N?(a) Rate-zonal centrifugation(b) Isopycnic centrifugation(c) HPLC(d) Mass spectrometryThis question was addressed to me in an interview for job.This question is from Nucleic-acid Techniques in section Techniques in Cell Biology of Cell Biology

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