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Which of the following is true about tm?(a) It can be termed as renaturation temperature(b) The higher the content of G ≡ C base pairs, the higher the tm(c) The higher the content of A = T base pairs, the higher the tm(d) The higher the content of G ≡ C base pairs, the lower the tmThe question was asked at a job interview.My question is from Nucleic Acid Chemistry topic in portion Nucleotides and Nucleic Acids of Biochemistry

Answer»

Right choice is (b) The higher the content of G ≡ C BASE PAIRS, the higher the TM

The best I can explain: Tm is the melting TEMPERATURE or denaturation temperature.



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