1.

You take a circular ssDNA and to it you attach a small labeled complimentary fragment. You add different reagents and try to get free labeled probe. Which of this reagent will give you your desired result?(a) Dna b(b) Dna c(c) Dna G(d) Dna aI have been asked this question during an online interview.My question comes from DNA Replication Prokaryotes topic in chapter DNA Replication of Cytogenetics

Answer»

Correct choice is (a) Dna b

For explanation I would say: Dna b has helicase activity which can separate TWO complementary DNA STRANDS, this will break the link between labeled fragment and circular DNA rendering it FREE. Dna G mainly has PRIMASE activity while Dna a and Dna c are required for replication initiation which don’t give the DESIRED result.



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