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Calculate the ratio of the concentrations of acetate and acetic acid required in a buffer system of pH 5.30.(a) 3.2(b) 3.3(c) 3.4(d) 3.5I have been asked this question during an interview.This interesting question is from Buffering against pH changes in Biological Systems topic in chapter Water Basics of Biochemistry

Answer»

The correct choice is (d) 3.5

For explanation: PH = PKA + LOG [acetate]/[acetic ACID]

log [acetate]/[acetic acid] = pH – pKa

= 5.30 – 4.76 = 0.54

[acetate]/[acetic acid] = ANTILOG 0.54 = 3.5.



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