

InterviewSolution
This section includes InterviewSolutions, each offering curated multiple-choice questions to sharpen your knowledge and support exam preparation. Choose a topic below to get started.
1. |
What is the DNA composition of ARS?(a) A-T rich(b) G-C rich(c) Repeating A-T, G-C sequence(d) No specific sequence specialityThe question was posed to me at a job interview.Origin of the question is DNA Replication Eukaryotes in portion DNA Replication of Cytogenetics |
Answer» The CORRECT option is (a) A-T rich |
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2. |
We know that Beta clamp in bacteria has two subunits and takes the shape of a ring. Which of this will be characteristic of PCNA which is a beta clamp homologue for eukaryotes?(a) Two subunits forming ring(b) Three subunits forming ring(c) Four subunits forming figure 8 like structure(d) Four subunits forming a larger ringI had been asked this question in final exam.Query is from DNA Replication Eukaryotes in chapter DNA Replication of Cytogenetics |
Answer» Correct ANSWER is (b) Three subunits FORMING ring |
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3. |
In an experiment, you are working on mitochondrial chromosomes. Which polymerase will ensure accurate replication?(a) pol beta(b) pol delta(c) pol iota(d) pol gammaI had been asked this question in class test.I would like to ask this question from DNA Replication Eukaryotes topic in portion DNA Replication of Cytogenetics |
Answer» CORRECT option is (d) POL gamma Best explanation: Pol gamma is the mitochondrial DNA polymerase, while pol beta is mainly necessary for repair. Pol iota ASSOCIATES with translesion DNA SYNTHESIS. Pol delta is the primary nuclear DNA polymerase. |
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4. |
What is the fate of phosphorylated Cdc 6?(a) It is transported outside nucleus(b) It is ubiquitinylated(c) It gets activated(d) It forms a complex with CdtThis question was addressed to me in exam.I'd like to ask this question from DNA Replication Eukaryotes in division DNA Replication of Cytogenetics |
Answer» Correct choice is (B) It is ubiquitinylated |
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5. |
Cyclin-CDK complex (here considering S cyclin) when active is responsible for phosphorylating several proteins and this often marks then for destruction by ubiquitination. What would be its effect on Cdt activity?(a) Activation(b) Inactivation(c) Destruction(d) Increased synthesisI have been asked this question in semester exam.My question is from DNA Replication Eukaryotes in division DNA Replication of Cytogenetics |
Answer» The CORRECT OPTION is (a) Activation |
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6. |
Which subunit in MCM is not phosphorylated in MCM after initiation is triggered?(a) 1(b) 2(c) 3(d) 7I have been asked this question during an internship interview.This intriguing question comes from DNA Replication Eukaryotes topic in division DNA Replication of Cytogenetics |
Answer» CORRECT option is (b) 2 For EXPLANATION I would SAY: MCM has 6 subunits numbered from 2 to 7. After INITIATION of replication is triggered all subunits other than 2 are phosphorylated. |
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7. |
Here are names of some factors necessary for prokaryotic replication. Which of these or their homologue is unnecessary for eukaryotes?(a) Dna G(b) Dna b(c) Beta clamp(d) SSBI got this question in an interview.The question is from DNA Replication Eukaryotes topic in chapter DNA Replication of Cytogenetics |
Answer» The correct choice is (a) Dna G |
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8. |
You design a plasmid with a gene for Histidine synthesis and a fragment containing ARS. You introduce it yeast culture and try to grow then in Histidine deficit media. What will be your observation?(a) No growth(b) One or two colonies(c) Rapid growth with many colonies(d) Normal growth with countable coloniesI have been asked this question in an interview.This is a very interesting question from DNA Replication Eukaryotes in chapter DNA Replication of Cytogenetics |
Answer» The correct CHOICE is (C) Rapid growth with many colonies |
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9. |
Which Cyclin-CDK complex will you find most active in M phase?(a) Cyclin A- CDK 2(b) Cyclin B- CDK 1(c) Cyclin D- CDK 2(d) Cyclin E- CDK 1The question was posed to me in homework.My question is from DNA Replication Eukaryotes in division DNA Replication of Cytogenetics |
Answer» RIGHT choice is (b) Cyclin B- CDK 1 The explanation: Cyclin B – CDK 1 is CORRESPONDING to the YEAST M-Cyclin-CDK complex which is active during M phase and leads to Anaphase. Cyclin D is G1-S cyclin, Cyclin A is S cyclin and Cyclin E is G1 cyclin respectively. |
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10. |
You are given a DNA fragment with a CBD lesion. Which polymerase(s) will you use to replicate this fragment without taking care of the error?(a) ζ (zeta), η (eta)(b) η (eta), ) θ (theta)(c) θ (theta), κ (kappa)(d) κ (kappa),θ (theta)The question was asked during an interview.I would like to ask this question from DNA Replication Eukaryotes topic in section DNA Replication of Cytogenetics |
Answer» The correct OPTION is (b) η (eta), ) θ (theta) |
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11. |
In case of eukaryotes replication initiates at ________(a) TATA(b) C^pG islets(c) AUG(d) ARSI had been asked this question during an internship interview.My question comes from DNA Replication Eukaryotes in portion DNA Replication of Cytogenetics |
Answer» Correct CHOICE is (d) ARS |
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12. |
Acyclovir is a drug used to treat viral infection by impairing its replication. Why will it not effect bacterial replication as well?(a) Bacteria under viral attack don’t replicate(b) Viral polymerase binds to it and thus can’t perform its function(c) Virus uses it in a polymerization(d) Cellular mechanism deactivates itI had been asked this question during a job interview.The above asked question is from DNA Replication Prokaryotes in chapter DNA Replication of Cytogenetics |
Answer» Correct ANSWER is (c) VIRUS uses it in a polymerization |
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13. |
You design a circular ssDNA with a labeled RNA primer (alpha-P^32 labeling). You add polymerase epsilon and required enzymes for replication to the fragment. What will you expect to see on autoradiography after gel electrophoresis?(a) Single labeled band with some labeling at the bottom region(b) Two labeled band one much lower than the other with labeling at the bottom region(c) No band but labeling at the bottom(d) Two labeled band one much lower than the other with no labeling at the bottomThis question was posed to me during an interview for a job.The query is from DNA Replication Eukaryotes topic in division DNA Replication of Cytogenetics |
Answer» Correct answer is (d) Two labeled band one much lower than the other with no labeling at the bottom |
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14. |
Bacterial polymerases are slower than eukaryotic polymerases.(a) True(b) FalseThe question was posed to me in an online interview.This is a very interesting question from DNA Replication Prokaryotes topic in chapter DNA Replication of Cytogenetics |
Answer» The CORRECT option is (b) False |
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15. |
What is the function of RFC in eukaryotic replication?(a) Catalytic subunit(b) SSB(c) Clamp loader(d) PrimaseThe question was asked in an interview for job.My enquiry is from DNA Replication Eukaryotes in portion DNA Replication of Cytogenetics |
Answer» CORRECT choice is (c) Clamp loader Best explanation: RFC or Replication FACTOR C is the EUKARYOTIC HOMOLOGUE of clamp loader. It loads the PCNA clamp. Eukaryotic replication primer is formed by alpha polymerase while RPA acts as SSB. |
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16. |
You wanted to find out the importance of the DNA polymerases. So, you mutated the polymerases one by one and checked for survival. In which of these mutant cases will you see least survival rate?(a) Alpha(b) Beta(c) Delta(d) EtaThis question was posed to me during an online exam.Question is taken from DNA Replication Eukaryotes topic in section DNA Replication of Cytogenetics |
Answer» Correct option is (a) ALPHA |
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17. |
You take a circular ssDNA and to it you attach a small labeled complimentary fragment. You add different reagents and try to get free labeled probe. Which of this reagent will give you your desired result?(a) Dna b(b) Dna c(c) Dna G(d) Dna aI have been asked this question during an online interview.My question comes from DNA Replication Prokaryotes topic in chapter DNA Replication of Cytogenetics |
Answer» Correct choice is (a) Dna b |
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18. |
In an experiment you take a DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?(a) Dna a, Dna b, HU, SSB, topoisomerase I, polymerase I(b) Dna a, Dna b, HU, SSB, polymerase III(c) Dna a, Dna b,Dna c, HU ,SSBpolymerase I(d) Dna a, Dna b, Dna c, HU, SSB, polymerase IIII have been asked this question by my school principal while I was bunking the class.I'd like to ask this question from DNA Replication Prokaryotes in portion DNA Replication of Cytogenetics |
Answer» Right option is (C) Dna a, Dna b,Dna c, HU ,SSBpolymerase I |
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19. |
Which is the most processive of prokaryotic DNA polymerases?(a) pol I(b) pol II(c) pol III(d) klenow fragmentI got this question during an interview.I'd like to ask this question from DNA Replication Prokaryotes in division DNA Replication of Cytogenetics |
Answer» Correct choice is (c) pol III |
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20. |
What will be the fate of a Seq A mutant E. Coli?(a) DNA replication will occur less frequently(b) Replication will be error prone(c) Replication will not occur(d) Uncontrolled replicationThis question was posed to me during an internship interview.I'd like to ask this question from DNA Replication Prokaryotes topic in chapter DNA Replication of Cytogenetics |
Answer» RIGHT choice is (b) Replication will be ERROR prone Explanation: SEQ A ( Seq standing for sequestering) blocks the newly produced DNA stand from Methylase. This PLAYS an important role in Mis-Match repair. In its absence Mis-match repair will be non functional producing erroneous DNA. |
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21. |
In an experiment you use DNA polI – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.(a) No difference from intact DNA pol I replication(b) Replication will be slower(c) Replication will be error prone(d) DNA produced will be shorterThis question was addressed to me in an interview for internship.I want to ask this question from DNA Replication Prokaryotes in division DNA Replication of Cytogenetics |
Answer» The correct OPTION is (d) DNA produced will be shorter |
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22. |
Deletion of which domain of ARS would give the least replication rate in eukaryotes?(a) A domain(b) B1 domain(c) B2 domain(d) B3 domainI got this question in unit test.I'd like to ask this question from DNA Replication Eukaryotes topic in chapter DNA Replication of Cytogenetics |
Answer» The CORRECT choice is (a) A domain |
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23. |
Which of this subunit is not a part of core DNA polymerase?(a) Alpha(b) Beta(c) Theta(d) EtaI have been asked this question at a job interview.Query is from DNA Replication Prokaryotes in portion DNA Replication of Cytogenetics |
Answer» Right CHOICE is (b) Beta |
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24. |
You grow a bacterial culture in a media containing N^15 and transfer it to a media with N^14. After two rounds of replication you perform a CsCl density gradient centrifugation of the DNA. How many bands will you observe what will be their intensity?(a) One, very intense(b) Two, equally intense(c) Three with middle one more intense than upper and lower(d) Three equally intenseThis question was addressed to me during an online interview.I'm obligated to ask this question of DNA Replication Prokaryotes topic in division DNA Replication of Cytogenetics |
Answer» Right OPTION is (b) TWO, equally intense |
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25. |
To your cultured cell lines, you add growth factors like cytokines which act through MAPK cascade pathway. Which transition will it trigger?(a) S->G2(b) M->Anaphase(c) G1->S(d) G2->MThe question was posed to me in an internship interview.My doubt stems from DNA Replication Eukaryotes topic in portion DNA Replication of Cytogenetics |
Answer» Correct answer is (c) G1->S |
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26. |
Pre-replication complex is necessary to initiate replication. It assembles in______________(a) Late Anaphase(b) G1 phase(c) S phase(d) Early AnaphaseThe question was posed to me in quiz.My doubt stems from DNA Replication Eukaryotes topic in portion DNA Replication of Cytogenetics |
Answer» Correct answer is (B) G1 PHASE |
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27. |
Which of these would you use as a competitive inhibitor for Cdt?(a) MCM 1(b) MCM 2(c) MCM 7(d) MCM 10I had been asked this question in homework.This intriguing question comes from DNA Replication Eukaryotes topic in section DNA Replication of Cytogenetics |
Answer» Correct answer is (d) MCM 10 |
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28. |
If we mutate the DNA ligase and observe the length of the replicated strands in different time slots after replication initiation, what will we observe?(a) The DNA will gradually increase in length till it is fully replicated(b) Small fragments of DNA will be obtained increasing in number with time(c) Mixture of small and long Fragments of definite length from the start whose concentration simply increases with time(d) At first small fragment, then two separate bands showing long fragment with increasing length and short fragments of definite length.I have been asked this question in class test.My question is based upon DNA Replication Prokaryotes topic in portion DNA Replication of Cytogenetics |
Answer» RIGHT CHOICE is (d) At first SMALL FRAGMENT, then two separate bands showing long fragment with increasing length and short fragments of definite length. Best explanation: The small fragments are the Okazaki fragments of lagging strand while the longer is the leading stand DNA. While leading strand grows in length the Okazaki fragments in absence of ligation to each other results in small fragments of definite length. |
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29. |
What constitutes Primosome?(a) Dna a, Dna b, Dna c, Dna G(b) Dna b, Dna G(c) Dna c, Dna b(d) Dna a, Dna cI had been asked this question in an online quiz.Query is from DNA Replication Prokaryotes topic in chapter DNA Replication of Cytogenetics |
Answer» CORRECT option is (b) DNA b, Dna G The best I can explain: Dna b, the HELICASE, and Dna G, the primase, constitutes the primosome NECESSARY for initiation. Dna a mainly generates a strain allowing Dna c to load the helicase Dna b. |
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30. |
At which end are the new DNA bases added?(a) 5’ triphosphate end(b) 3’ triphosphate end(c) 5’ OH end(d) 3’ OH endThis question was addressed to me in homework.The origin of the question is DNA Replication Prokaryotes topic in chapter DNA Replication of Cytogenetics |
Answer» CORRECT option is (d) 3’ OH end Easy explanation: DNA replication proceeds from 5’ -> 3’ direction with new bases being added to 3’-OH end. 5’-P is at the beginning of DNA STRAND. |
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31. |
How many prokaryotic DNA polymerases have 5’->3’ proofreading activity?(a) 1(b) 2(c) 3(d) 4I had been asked this question in a national level competition.This interesting question is from DNA Replication Prokaryotes topic in chapter DNA Replication of Cytogenetics |
Answer» The correct answer is (a) 1 |
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32. |
Rolling circle mode of replication is ________(a) Conservative(b) Non Conservative(c) Semi – Conservative(d) DispersedI had been asked this question during an interview.My query is from DNA Replication Prokaryotes topic in chapter DNA Replication of Cytogenetics |
Answer» Correct answer is (c) Semi – Conservative |
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